• RETRACTED: Tomassoni et al. Brain Activity of Thioctic Acid Enantiomers: In Vitro and In Vivo Studies in an Animal Model of Cerebrovascular Injury. <em>Int. J. Mol. Sci.</em> 2013, <em>14</em>, 4580-4595 - (le 27/05/2026)

    The journal retracts the article, "Brain Activity of Thioctic Acid Enantiomers: In Vitro and in Vivo Studies in an Animal Model of Cerebrovascular Injury" [...].

    • Daniele Tomassoni, Francesco Amenta, Consuelo Amantini, Valerio Farfariello, Lorenzo Di Cesare Mannelli, Innocent E Nwankwo, Carlotta Marini, Seyed Khosrow Tayebati

  • Reduced store operated calcium entry contributes to autophagy mediated escape of prostate cancer to oxaliplatin treatment - (le 06/04/2025)

    Oxaliplatin, a third-generation platinum-based chemotherapeutic drug, induces cell cycle arrest and apoptosis in prostate cancer treatment. However, both intrinsic and acquired resistance mechanisms limit its therapeutic efficacy. Notably, chemotherapeutic agents often induce autophagy-a cellular recycling process-that can contribute to drug resistance. Calcium (Ca^(2+)) signalling plays a pivotal role in regulating cell fate. However, the involvement of Ca^(2+) and Ca^(2+) channels in...

    • Dheeraj Kannancheri Puthooru, Maya Yassine, Dmitri Gordienko, Nathalie Ziental-Gelus, Emilie Desruelles, Valerio Farfariello, Loïc Lemonnier, Natalia Prevarskaya

  • Converting cell death into senescence by PARP1 inhibition improves recovery from acute oxidative injury - (le 09/05/2024)

    Excessive amounts of reactive oxygen species (ROS) lead to macromolecular damage and high levels of cell death with consequent pathological sequelae. We hypothesized that switching cell death to a tissue regenerative state could potentially improve the short-term and long-term detrimental effects of ROS-associated acute tissue injury, although the mechanisms regulating oxidative stress-induced cell fate decisions and their manipulation for improving repair are poorly understood. Here, we show...

    • Jamil Nehme, Lina Mesilmany, Marta Varela-Eirin, Simone Brandenburg, Abdullah Altulea, Yao Lin, Mariana Gaya da Costa, Marc Seelen, Jan-Luuk Hillebrands, Harry van Goor, Raya Saab, Haidar Akl, Natacha Prevarskaya, Valerio Farfariello, Marco Demaria

  • Correction to: The context‑dependent role of the Na<sup>+</sup>/Ca<sup>2+</sup>‑exchanger (NCX) in pancreatic stellate cell migration - (le 03/11/2023)

    No abstract

    • Thorsten Loeck, Micol Rugi, Luca Matteo Todesca, Paulina Kalinowska, Benjamin Soret, Ilka Neumann, Sandra Schimmelpfennig, Karolina Najder, Zoltán Pethő, Valerio Farfariello, Natalia Prevarskaya, Albrecht Schwab

  • The context-dependent role of the Na<sup>+</sup>/Ca<sup>2+</sup>-exchanger (NCX) in pancreatic stellate cell migration - (le 11/08/2023)

    Pancreatic stellate cells (PSCs) that can co-metastasize with cancer cells shape the tumor microenvironment (TME) in pancreatic ductal adenocarcinoma (PDAC) by producing an excessive amount of extracellular matrix. This leads to a TME characterized by increased tissue pressure, hypoxia, and acidity. Moreover, cells within the tumor secrete growth factors. The stimuli of the TME trigger Ca^(2+) signaling and cellular Na^(+) loading. The Na^(+)/Ca^(2+) exchanger (NCX) connects the cellular Ca^(2+)...

    • Thorsten Loeck, Micol Rugi, Luca Matteo Todesca, Paulina Kalinowska, Benjamin Soret, Ilka Neumann, Sandra Schimmelpfennig, Karolina Najder, Zoltán Pethő, Valerio Farfariello, Natalia Prevarskaya, Albrecht Schwab

  • Acidic Growth Conditions Promote Epithelial-to-Mesenchymal Transition to Select More Aggressive PDAC Cell Phenotypes In Vitro - (le 13/05/2023)

    Pancreatic Ductal Adenocarcinoma (PDAC) is characterized by an acidic microenvironment, which contributes to therapeutic failure. So far there is a lack of knowledge with respect to the role of the acidic microenvironment in the invasive process. This work aimed to study the phenotypic and genetic response of PDAC cells to acidic stress along the different stages of selection. To this end, we subjected the cells to short- and long-term acidic pressure and recovery to pH(e) 7.4. This treatment...

    • Madelaine Magalì Audero, Tiago Miguel Amaral Carvalho, Federico Alessandro Ruffinatti, Thorsten Loeck, Maya Yassine, Giorgia Chinigò, Antoine Folcher, Valerio Farfariello, Samuele Amadori, Chiara Vaghi, Albrecht Schwab, Stephan J Reshkin, Rosa Angela Cardone, Natalia Prevarskaya, Alessandra Fiorio Pla

  • Involvement of ORAI1/SOCE in Human AML Cell Lines and Primary Cells According to ABCB1 Activity, LSC Compartment and Potential Resistance to Ara-C Exposure - (le 28/05/2022)

    Acute myeloid leukemia (AML) is a hematological malignancy with a high risk of relapse. This issue is associated with the development of mechanisms leading to drug resistance that are not yet fully understood. In this context, we previously showed the clinical significance of the ATP binding cassette subfamily B-member 1 (ABCB1) in AML patients, namely its association with stemness markers and an overall worth prognosis. Calcium signaling dysregulations affect numerous cellular functions and are...

    • Clara Lewuillon, Aurélie Guillemette, Sofia Titah, Faruk Azam Shaik, Nathalie Jouy, Ossama Labiad, Valerio Farfariello, Marie-Océane Laguillaumie, Thierry Idziorek, Adeline Barthélémy, Pauline Peyrouze, Céline Berthon, Mehmet Cagatay Tarhan, Meyling Cheok, Bruno Quesnel, Loïc Lemonnier, Yasmine Touil

  • TRPC3 shapes the ER-mitochondria Ca<sup>2+</sup> transfer characterizing tumour-promoting senescence - (le 18/02/2022)

    Cellular senescence is implicated in a great number of diseases including cancer. Although alterations in mitochondrial metabolism were reported as senescence drivers, the underlying mechanisms remain elusive. We report the mechanism altering mitochondrial function and OXPHOS in stress-induced senescent fibroblasts. We demonstrate that TRPC3 protein, acting as a controller of mitochondrial Ca^(2+) load via negative regulation of IP(3) receptor-mediated Ca^(2+) release, is down regulated in...

    • Valerio Farfariello, Dmitri V Gordienko, Lina Mesilmany, Yasmine Touil, Emmanuelle Germain, Ingrid Fliniaux, Emilie Desruelles, Dimitra Gkika, Morad Roudbaraki, George Shapovalov, Lucile Noyer, Mathilde Lebas, Laurent Allart, Nathalie Zienthal-Gelus, Oksana Iamshanova, Franck Bonardi, Martin Figeac, William Laine, Jerome Kluza, Philippe Marchetti, Bruno Quesnel, Daniel Metzger, David Bernard, Jan B Parys, Loïc Lemonnier, Natalia Prevarskaya

  • ORAI3 silencing alters cell proliferation and promotes mitotic catastrophe and apoptosis in pancreatic adenocarcinoma - (le 02/04/2021)

    Changes in cytosolic free Ca^(2+) concentration play a central role in many fundamental cellular processes including muscle contraction, neurotransmission, cell proliferation, differentiation, gene transcription and cell death. Many of these processes are known to be regulated by store-operated calcium channels (SOCs), among which ORAI1 is the most studied in cancer cells, leaving the role of other ORAI channels yet inadequately addressed. Here we demonstrate that ORAI3 channels are expressed in...

    • Charlotte Dubois, Kateryna Kondratska, Artem Kondratskyi, Angela Morabito, Lina Mesilmany, Valerio Farfariello, Robert-Alain Toillon, Nathalie Ziental Gelus, Emilie Laurenge, Fabien Vanden Abeele, Loic Lemonnier, Natalia Prevarskaya

  • Calcium channel ITPR2 and mitochondria-ER contacts promote cellular senescence and aging - (le 02/02/2021)

    Cellular senescence is induced by stresses and results in a stable proliferation arrest accompanied by a pro-inflammatory secretome. Senescent cells accumulate during aging, promoting various age-related pathologies and limiting lifespan. The endoplasmic reticulum (ER) inositol 1,4,5-trisphosphate receptor, type 2 (ITPR2) calcium-release channel and calcium fluxes from the ER to the mitochondria are drivers of senescence in human cells. Here we show that Itpr2 knockout (KO) mice display improved...

    • Dorian V Ziegler, David Vindrieux, Delphine Goehrig, Sara Jaber, Guillaume Collin, Audrey Griveau, Clotilde Wiel, Nadia Bendridi, Sophia Djebali, Valerio Farfariello, Natacha Prevarskaya, Léa Payen, Jacqueline Marvel, Sébastien Aubert, Jean-Michel Flaman, Jennifer Rieusset, Nadine Martin, David Bernard

  • Ion Channel Profiling in Prostate Cancer: Toward Cell Population-Specific Screening - (le 02/08/2020)

    In the last three decades, a growing number of studies have implicated ion channels in all essential processes of prostate carcinogenesis, including cell proliferation, apoptosis, migration, and angiogenesis. The changes in the expression of individual ion channels show a specific profile, making these proteins promising clinical biomarkers that may enable better molecular subtyping of the disease and lead to more rapid and accurate clinical decision-making. Expression profiles and channel...

    • Valerio Farfariello, Natalia Prevarskaya, Dimitra Gkika

  • Co-targeting Mitochondrial Ca<sup>2+</sup> Homeostasis and Autophagy Enhances Cancer Cells' Chemosensitivity - (le 26/06/2020)

    Mitochondria are important cell death checkpoints, and mitochondrial Ca^(2+) overload is considered as a potent apoptotic intrinsic pathway inducer. Here, we report that this Ca^(2+) apoptosis link is largely ineffective in inducing cell-death just by itself and required a concomitant inhibition of autophagy to counteract its pro-survival action. In such condition, an acute mitochondrial stress revealed by a DRP1-mediated mitochondrial dynamic remodeling is observed concomitantly with...

    • Charlotte Dubois, Artem Kondratskyi, Gabriel Bidaux, Lucile Noyer, Eric Vancauwenberghe, Valério Farfariello, Robert-Allain Toillon, Morad Roudbaraki, Dominique Tierny, Jean-Louis Bonnal, Natalia Prevarskaya, Fabien Vanden Abeele

  • 4TM-TRPM8 channels are new gatekeepers of the ER-mitochondria Ca<sup>2+</sup> transfer - (le 22/04/2018)

    Calcium (Ca^(2+)) release from the endoplasmic reticulum plays an important role in many cell-fate defining cellular processes. Traditionally, this Ca^(2+) release was associated with the ER Ca^(2+) release channels, inositol 1,4,5‑triphosphate receptor (IP(3)R) and ryanodine receptor (RyR). Lately, however, other calcium conductances have been found to be intracellularly localized and to participate in cell fate regulation. Nonetheless, molecular identity and functional properties of the ER...

    • Gabriel Bidaux, Dmitri Gordienko, George Shapovalov, Valerio Farfariello, Anne-Sophie Borowiec, Oksana Iamshanova, Loic Lemonnier, Maxime Gueguinou, Roseline Guibon, Gaelle Fromont, Mélanie Paillard, Yves Gouriou, Christophe Chouabe, Etienne Dewailly, Dimitra Gkika, Pilar López-Alvarado, J Carlos Menéndez, Laurent Héliot, Christian Slomianny, Natalia Prevarskaya

  • The TRPV1 ion channel regulates thymocyte differentiation by modulating autophagy and proteasome activity - (le 07/12/2017)

    Autophagy and the ubiquitin-proteasome system (UPS) control thymus cell homeostasis under resting and endoplasmic reticulum (ER) stress conditions. Several evidence support a cross-talk between UPS and autophagy; abrogation of UPS responses stimulates autophagy, and vice versa the inhibition of autophagy alters the UPS functions. Herein, we found that TRPV1 activation induces ER stress, proteasome dysfunction and autophagy in thymocytes by modulating the expression of UPR-related genes. The...

    • Consuelo Amantini, Valerio Farfariello, Claudio Cardinali, Maria Beatrice Morelli, Oliviero Marinelli, Massimo Nabissi, Matteo Santoni, Laura Bonfili, Valentina Cecarini, Anna Maria Eleuteri, Giorgio Santoni

  • TRPs and Ca<sup>2+</sup> in cell death and survival - (le 02/08/2017)

    Transient Receptor Potential (TRP) family mediate the influx of monovalent and/or divalent cations into cells in response to environmental stimuli. Pharmacological or genetic manipulations of TRP channels demonstrate that TRP channels influence cell death rates, prolonging or shortening of cell survival. Due to their diverse cellular localization, TRP channels mediated Ca^(2+) influx generates distinct intracellular Ca^(2+) signals that regulate downstream pathways converging to apoptosis or...

    • Ingrid Fliniaux, Emmanuelle Germain, Valerio Farfariello, Natalia Prevarskaya

  • Sorafenib induces cathepsin B-mediated apoptosis of bladder cancer cells by regulating the Akt/PTEN pathway. The Akt inhibitor, perifosine, enhances the sorafenib-induced cytotoxicity against bladder cancer cells - (le 23/06/2015)

    Sorafenib, a tyrosine kinase inhibitor, has been demonstrated to exert anti-tumor effects. However, the molecular mechanisms underlying its effects on bladder cancer remain unknown. Here, we evaluated the mechanisms responsible for the sorafenib-induced anti-tumor effects on 5637 and T24 bladder cancer cells. We demonstrated that sorafenib reduces cell viability, stimulates lysosome permeabilization and induces apoptosis of bladder cancer cells. These effects are dependent by the activation of...

    • Consuelo Amantini, Maria Beatrice Morelli, Matteo Santoni, Alessandra Soriani, Claudio Cardinali, Valerio Farfariello, Anna Maria Eleuteri, Laura Bonfili, Matteo Mozzicafreddo, Massimo Nabissi, Stefano Cascinu, Giorgio Santoni

  • Calcium homeostasis in cancer: A focus on senescence - (le 14/03/2015)

    Senescence is one of the primary responses to the activation of oncoproteins or down-regulation of tumor suppressors in normal cells and is therefore considered as being anti-tumorigenic but the mechanisms controlling this process are still much unknown. Calcium (Ca²⁺) plays a major role in many cellular processes and calcium channels control many of the "hallmarks of cancer" but their involvement in tumor initiation is poorly understood and remains unclear. Therefore, in this article we review...

    • Valerio Farfariello, Oksana Iamshanova, Emmanuelle Germain, Ingrid Fliniaux, Natalia Prevarskaya

  • Cross-talk between alpha1D-adrenoceptors and transient receptor potential vanilloid type 1 triggers prostate cancer cell proliferation - (le 08/12/2014)

    CONCLUSIONS: We demonstrate a cross-talk between alpha1D-AR and TRPV1, that is involved in the control of PC3 cell proliferation. These data strongly support for a putative novel pharmacological approach in the treatment of PCa by targeting both alpha1D-AR and TRPV1 channels.

    • Maria Beatrice Morelli, Consuelo Amantini, Massimo Nabissi, Sonia Liberati, Claudio Cardinali, Valerio Farfariello, Daniele Tomassoni, Wilma Quaglia, Alessandro Piergentili, Alessandro Bonifazi, Fabio Del Bello, Matteo Santoni, Gabriele Mammana, Lucilla Servi, Alessandra Filosa, Angela Gismondi, Giorgio Santoni

  • Resiniferatoxin induces death of bladder cancer cells associated with mitochondrial dysfunction and reduces tumor growth in a xenograft mouse model - (le 03/12/2014)

    Bladder cancer (BC) is the fifth most common non-cutaneous malignancy and the most common form of BC in Western countries is transitional cell carcinoma. Resiniferatoxin (RTX) has found therapeutic usefulness for the treatment of bladder dysfunction but no data are available on its use as chemotherapeutic agent. The aim of this work is to evaluate the use of RTX as new anti-cancer drug in BC therapy. The effects of RTX on cell viability and cell death were evaluated on T24 and 5637 BC cell lines...

    • Valerio Farfariello, Sonia Liberati, Maria Beatrice Morelli, Daniele Tomassoni, Matteo Santoni, Massimo Nabissi, Antonella Giannantoni, Giorgio Santoni, Consuelo Amantini

  • Loss of TRPV2 Homeostatic Control of Cell Proliferation Drives Tumor Progression - (le 09/04/2014)

    Herein we evaluate the involvement of the TRPV2 channel, belonging to the Transient Receptor Potential Vanilloid channel family (TRPVs), in development and progression of different tumor types. In normal cells, the activation of TRPV2 channels by growth factors, hormones, and endocannabinoids induces a translocation of the receptor from the endosomal compartment to the plasma membrane, which results in abrogation of cell proliferation and induction of cell death. Consequently, loss or...

    • Sonia Liberati, Maria Beatrice Morelli, Consuelo Amantini, Valerio Farfariello, Matteo Santoni, Alessandro Conti, Massimo Nabissi, Stefano Cascinu, Giorgio Santoni

  • Expression and function of the transient receptor potential ion channel family in the hematologic malignancies - (le 19/07/2013)

    Transient receptor potential (TRP) channels are important candidates mediating Ca(2+) influx in excitable and nonexcitable cells such as normal and neoplastic hematopoietic tissues. They are non selective cation channels implicated in Ca(2+) signaling in hematologic tumor cells. Here, we review the growing experimental evidence indicating that TRP channels should be included among the genes whose expression is altered in hematologic malignancies such as leukemias (AML, ALL, CML and CLL), B- and...

    • Maria B Morelli, Sonia Liberati, Consuelo Amantini, Massimo Nabiss, Matteo Santoni, Valerio Farfariello, Giorgio Santoni

  • Brain activity of thioctic Acid enantiomers: in vitro and in vivo studies in an animal model of cerebrovascular injury - (le 28/02/2013)

    Oxidative stress is an imbalance between the production of free radicals and antioxidant defense mechanisms, potentially leading to tissue damage. Oxidative stress has a key role in the development of cerebrovascular and/or neurodegenerative diseases. This phenomenon is mainly mediated by an enhanced superoxide production by the vascular endothelium with its consequent dysfunction. Thioctic, also known as alpha-lipoic acid (1,2-dithiolane-3-pentanoic acid), is a naturally occurring antioxidant...

    • Daniele Tomassoni, Francesco Amenta, Consuelo Amantini, Valerio Farfariello, Lorenzo Di Cesare Mannelli, Innocent E Nwankwo, Carlotta Marini, Seyed Khosrow Tayebati

  • The role of transient receptor potential vanilloid type-2 ion channels in innate and adaptive immune responses - (le 20/02/2013)

    The transient receptor potential vanilloid type-2 (TRPV2), belonging to the transient receptor potential channel family, is a specialized ion channel expressed in human and other mammalian immune cells. This channel has been found to be expressed in CD34(+) hematopoietic stem cells, where its cytosolic Ca(2) (+) activity is crucial for stem/progenitor cell cycle progression, growth, and differentiation. In innate immune cells, TRPV2 is expressed in granulocytes, macrophages, and monocytes where...

    • Giorgio Santoni, Valerio Farfariello, Sonia Liberati, Maria B Morelli, Massimo Nabissi, Matteo Santoni, Consuelo Amantini

  • Structure-activity relationships in 1,4-benzodioxan-related compounds. 11. (1) reversed enantioselectivity of 1,4-dioxane derivatives in α1-adrenergic and 5-HT1A receptor binding sites recognition - (le 21/12/2012)

    5-HT(1A) receptor and α(1)-adrenoreceptor (α(1)-AR) binding sites recognized by the 1,4-dioxanes 2-4 display reversed stereochemical requirements. (S)-2 proved to be a potent 5-HT(1A) receptor agonist highly selective over α(1)-AR subtypes. Chirality influenced the anticancer activity of 3 and 4 in human prostate cancer cells (PC-3): (R)-4, eutomer at the α(1d)-AR subtype, was the most potent. The decreased effect of 4 and (R)-4 in α(1d)-AR silenced PC-3 cells confirmed that their anticancer...

    • Alessandro Bonifazi, Alessandro Piergentili, Fabio Del Bello, Yogita Farande, Mario Giannella, Maria Pigini, Consuelo Amantini, Massimo Nabissi, Valerio Farfariello, Giorgio Santoni, Elena Poggesi, Amedeo Leonardi, Sergio Menegon, Wilma Quaglia

  • Onabotulinumtoxin-A intradetrusorial injections modulate bladder expression of NGF, TrkA, p75 and TRPV1 in patients with detrusor overactivity - (le 19/12/2012)

    How onabotulinumtoxinA (onab/A) injected in the detrusor muscle improves detrusor overactivity (DO) is still a matter of debate. Nerve growth factor (NGF) seems to play a role in determining urgency and DO. Recent studies showed that NGF decreases in patients with DO who respond to onab/A treatment. We investigated onab/A-induced changes on gene expression of NGF, TRPV1, TrkA and p75 in bladder wall tissue of patients affected by neurogenic and idiopathic DO. Twenty-five patients (18 with...

    • Antonella Giannantoni, Antonella Conte, Valerio Farfariello, Silvia Proietti, Alberto Vianello, Vincenza Nardicchi, Giorgio Santoni, Consuelo Amantini

  • Transient receptor potential vanilloid 1 activation induces autophagy in thymocytes through ROS-regulated AMPK and Atg4C pathways - (le 04/07/2012)

    Autophagy is a highly conserved process involved in lymphocyte development and differentiation. Herein, we demonstrated for the first time that triggering of TRPV1 by the specific agonist CPS induces autophagy in mouse thymocytes. TRPV1-dependent autophagy required [Ca(2+)](i) and ROS generation, resulting in AMPK activation. CPS specifically increased Atg4C mRNA expression and induced oxidation of Atg4C protein by ROS generation. TRPV1-triggered autophagy was Atg6/Beclin-1-dependent, as...

    • Valerio Farfariello, Consuelo Amantini, Giorgio Santoni

  • Present and future of tyrosine kinase inhibitors in renal cell carcinoma: analysis of hematologic toxicity - (le 29/05/2012)

    Tyrosine kinase inhibitors (TKIs) have dramatically improved the outcome of renal cell carcinoma (RCC) patients. The use of these agents requires early and appropriate management of side effects such as hematologic adverse events (HAE), in order to avoid unnecessary dose reductions and transitory or definitive treatment discontinuations. Beyond the increased infective risk, myelosuppression contributes to TKI-related fatigue, thus reducing both patients' quality of life and overall survival...

    • Matteo Santoni, Mimma Rizzo, Luciano Burattini, Valerio Farfariello, Rossana Berardi, Giorgio Santoni, Giacomo Carteni, Stefano Cascinu

  • Antioncogenic effects of transient receptor potential vanilloid 1 in the progression of transitional urothelial cancer of human bladder - (le 24/04/2012)

    The progression of normal cells to a tumorigenic and metastatic state involves the accumulation of mutations in multiple key signaling proteins, encoded by oncogenes and tumor suppressor genes. Recently, members of the TRP channel family have been included in the oncogenic and tumor suppressor protein family. TRPM1, TRPM8, and TRPV6 are considered to be tumor suppressors and oncogenes in localized melanoma and prostate cancer, respectively. Herein, we focus our attention on the antioncogenic...

    • Giorgio Santoni, Sara Caprodossi, Valerio Farfariello, Sonia Liberati, Angela Gismondi, Consuelo Amantini

  • The transient receptor potential vanilloid-2 cation channel impairs glioblastoma stem-like cell proliferation and promotes differentiation - (le 12/04/2012)

    Malignant transformation of cells resulting from enhanced proliferation and aberrant differentiation is often accompanied by changes in transient receptor potential vanilloid (TRPV) channels expression. In gliomas, recent evidence indicates that TRPV type 2 (TRPV2) negatively controls glioma cell survival and proliferation. In addition, cannabinoids, the ligands of both cannabinoid and TRPV2 receptors, promote glioblastoma stem-like cells (GSCs) differentiation and inhibit gliomagenesis. Herein,...

    • Maria Beatrice Morelli, Massimo Nabissi, Consuelo Amantini, Valerio Farfariello, Lucia Ricci-Vitiani, Simona di Martino, Roberto Pallini, Luigi Maria Larocca, Sara Caprodossi, Matteo Santoni, Ruggero De Maria, Giorgio Santoni

  • 4-nonylphenol triggers apoptosis and affects 17-β-estradiol receptors in calvarial osteoblasts - (le 08/11/2011)

    The present research examines the effects of 4-nonylphenol (4-NP) on mouse primary calvarial osteoblasts (COBs). Incubation of the cells with 4-NP at 10(-5)M and 10(-6)M striking decreased osteoblasts viability and phosphatidylserine (PS) exposure, measured by Annexin V, was greatly enhanced. In addition, an up-regulation of Bax/Bcl2 ratio with a drop in ΔΨm and an increase of cleaved caspase 9 and 3 was found, suggesting that the alkylphenol induced osteoblast death via the...

    • Maria Giovanna Sabbieti, Dimitrios Agas, Francesco Palermo, Gilberto Mosconi, Giorgio Santoni, Consuelo Amantini, Valerio Farfariello, Luigi Marchetti

  • IL-22 mRNA in peripheral blood mononuclear cells from allergic rhinitic and asthmatic pediatric patients - (le 04/05/2011)

    T helper (T(H) )-17 lymphocytes are characterized by the expression of many regulatory cytokines, including IL-17A and IL-22, but at present no clinical data are available on the expression of these cytokines in peripheral blood mononuclear cells (PBMC) from allergic asthmatic and rhinitic (AR) children. The aim of this study was to investigate a possible relationship between IL-22 and IL-17A mRNAs and clinical parameters in seroatopic, AR, and asthmatic children. The study, conducted during the...

    • Valerio Farfariello, Consuelo Amantini, Massimo Nabissi, Maria Beatrice Morelli, Cristiana Aperio, Sara Caprodossi, Antonio Carlucci, Anna Maria Bianchi, Giorgio Santoni

  • TRP channels and cancer: new targets for diagnosis and chemotherapy - (le 26/02/2011)

    The Transient Receptor Potential (TRP) channels family consists of seven different subfamilies, namely TRPC (Canonical), TRPV (Vanilloid), TRPM (Melastatin), TRPML (Mucolipin), TRPP (Polycystin), and TRPA (Ankyrin transmembrane protein) and TRPN (NomPC-like) that are related to several physiological and pathological processes. Recent years have witnessed an increased interest of research into the connection between TRP channels and cancer, leading to the discovery of tumor-related functions such...

    • Giorgio Santoni, Valerio Farfariello

  • Capsaicin promotes a more aggressive gene expression phenotype and invasiveness in null-TRPV1 urothelial cancer cells - (le 12/02/2011)

    Capsaicin (CPS) has been found to exhibit either tumor promoting or suppressing effects, many of which are mediated by the specific transient receptor potential vanilloid type-1 (TRPV1). Herein, we provide evidence that CPS treatment induced a more aggressive gene phenotype and invasiveness in 5637 cells-lacking TRPV1 receptor. CPS treatment of 5637 cells induced upregulation of pro-angiogenetic (angiopoietin 1, angiopoietin 2 and vascular endothelial growth factor), pro-invasive and...

    • Sara Caprodossi, Consuelo Amantini, Massimo Nabissi, Maria Beatrice Morelli, Valerio Farfariello, Matteo Santoni, Angela Gismondi, Giorgio Santoni

  • TRPV channels in tumor growth and progression - (le 04/02/2011)

    Transient receptor potential (TRP) channels affect several physiological and pathological processes. In particular, TRP channels have been recently involved in the triggering of enhanced proliferation, aberrant differentiation, and resistance to apoptotic cell death leading to the uncontrolled tumor invasion. About thirty TRPs have been identified to date, and are classified in seven different families: TRPC (Canonical), TRPV (Vanilloid), TRPM (Melastatin), TRPML (Mucolipin), TRPP (Polycystin),...

    • Giorgio Santoni, Valerio Farfariello, Consuelo Amantini

  • Expression of transient receptor potential vanilloid-1 (TRPV1) in urothelial cancers of human bladder: relation to clinicopathological and molecular parameters - (le 19/11/2010)

    CONCLUSIONS: The progression of UC of human bladder is associated with a marked decrease in TRPV1 expression, with a progressive loss in high-grade muscle invasive UC. Downregulation of TRPV1 mRNA expression may represent an independent negative prognostic factor for bladder cancer patients.

    • Cristina Kalogris, Sara Caprodossi, Consuelo Amantini, Federica Lambertucci, Massimo Nabissi, Maria Beatrice Morelli, Valerio Farfariello, Alessandra Filosa, Maria Cristina Emiliozzi, Gabriele Mammana, Giorgio Santoni

  • TRPV2 channel negatively controls glioma cell proliferation and resistance to Fas-induced apoptosis in ERK-dependent manner - (le 23/01/2010)

    The aim of this study was to investigate the expression and function of the transient receptor potential vanilloid 2 (TRPV2) in human glioma cells. By Real-Time-PCR and western blot analysis, we found that TRPV2 messenger RNA (mRNA) and protein were expressed in benign astrocyte tissues, and its expression progressively declined in high-grade glioma tissues as histological grade increased (n = 49 cases), and in U87MG cells and in MZC, FCL and FSL primary glioma cells. To investigate the function...

    • Massimo Nabissi, Maria Beatrice Morelli, Consuelo Amantini, Valerio Farfariello, Lucia Ricci-Vitiani, Sara Caprodossi, Antonella Arcella, Matteo Santoni, Felice Giangaspero, Ruggero De Maria, Giorgio Santoni